rLaminin -521 (Human) | Recombinant Heterotrimer | Corning

Corning® rLaminin-521 (human) is an animal-component-free recombinant heterotrimer composed of α5, β2, and γ1 chains expressed in a mammalian cell culture system. rLaminin-521 (Human) supports long-term self-renewal of human pluripotent stem cells (hPSC), including embryonic stem cells (hESC) and induced pluripotent stemcells (iPSC) in defined and xeno-free environments. rLaminin-521 provides additional benefits, including ROCK inhibitor independent single cell expansion of PSCs, which improves hPSC culture ease and efficiency.

Corning rlaminin-521 is available in vialed formats as well as pre-coated 6-well plates.

Through a collaboration between Corning and BioLamina, we have brought together expertise in rLaminin-521 expression with Corning’s proprietary surface coating technology. The result is Corning® PureCoat™ rLaminin-521 6-well plates. This precoated solution enables ROCK-inhibitor independent single cell passaging, which improves culture consistency and lowers contamination risk compared to labor-intensive clump/colony passaging methods.

From cell seeding techniques and density recommendations to the most effective types of media culture to use with this surface, this FAQ answers some of the most commonly asked questions about how to optimize cell culture with Corning rLaminin-521 (Human).

Get answers to commonly asked questions for PureCoat rLaminin-521 6-well plates.

Read how stem cell scientists efficiently scale-up their cell production in a xeno-free culture environment.  

This study demonstrates the undifferentiated expansion of hNSCs on Corning rLaminin-521 (Human).

Corning has partnered with BioLamina for the supply of recombinant human laminin 521 (rLaminin-521), a next generation surface that enables feeder-free, single cell passaging of pluripotent stem cells (PSCs) without ROCK-inhibitor. Now we've gone one step further and are working together to combine BioLamina’s expertise in recombinant laminin expression with Corning’s extensive line of vessels and proprietary surface coating technologies. This partnership brings robust and scalable expansion solutions to the emerging stem cell processing market, enabling our customers to scale-up effectively and mitigate risk.

Now backed by Corning’s large network, worldwide distribution channels, and cell culture portfolio, researchers globally have better access and availability to rLaminin-521 (Human) than ever before. 

rLaminin-521 (Human) joins the Corning ECM mimetic and advanced surfaces family: animal component-free surfaces suited for stem and progenitor cell expansion under cGMP processing environments.