Improve cell attachment by using cell culture surfaces coated with Corning human fibronectin (HFN) surface. HFN is produced by a wide variety of mesenchymal and epithelial cells, and is present in both the ECM and plasma. Cell adhesion to fibronectin is mediated by the central cell-binding domain of FN through RGD (Arg-Gly-Asp) amino acid sequence. The principal functions of fibronectin appear to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis.
Overview
Fibronectin
Characteristics and Applications
Applications
| Suitable as a thin coating on tissue culture surfaces to promote attachment, spreading and proliferation of a variety of cell types. It can also be used as an additive to serum-free culture medium.
Specific applications include: • Integrin and cell-surface HSPG-mediated binding of fibronectin-null fibroblasts
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Source
| Human plasma
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Protein Concentration | Lyophilized (100 mM CAPS, 0.15M NaCl, 1 mM CaCl2, pH 11.0).Reconstitute at 1 mg/mL
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Shelf Life | 2 years from date of manufacture. Date of expiration is located on lot specific certificate of analysis.
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Vialed Formats (Cat. No./Qty.) | 354008 | 1 mg 356008 | 5 mg 356009 | 25 mg (5 x 5 mg) |
BioCoatTM Options | Multiwell Plates: 6-well, 12-well, 24-well, 48-well, 96-well, 384-well[D1] . Dishes: 35 mm, 60 mm, 100 mm, 150 mm Inserts: for 6-well, 24-well, 96-well plates Coverslips: 22 mm Culture Slides: 4-well, 8-well Flasks: T-25, T-75, T-150, T-175 |
Quality Control
Quality Control
• Purity > 90% by SDS-PAGE
• Tested for ability to promote attachment and spreading of BHK-21 cells
• Tested and found negative for the presence of bacteria, fungi, and mycoplasma
• Purity > 90% by SDS-PAGE
• Tested for ability to promote attachment and spreading of BHK-21 cells
• Tested and found negative for the presence of bacteria, fungi, and mycoplasma
Primary Cells
| Aortic endothelial cells, BAEC Brain microvessel (endothelial) Cardiomyocytes; cardiac (endothelium, progenitor cells) Endothelial Cells; endothelial colony forming cells Hippocampal neurons HUVEC (endothelial) Keratinocytes Microvascular, BME (endothelial)
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Cell Lines (Transformed or Transfected)
| ARH-77 (lymphoblast) BHK-21 (fibroblast) COS-7 (fibroblast, transfected) 1299 (transfected- human non-small cell lung carcinoma cell line) hFOB 1.19, MG63 (osteoblast cell lines) Keratinocytes (human neonatal) MCF7 (epithelial) MCF-10A (epithelial) MDA-MB-231 NIH/3T3, 3T3 (fibroblast) SK-MEL-28 U266 (lymphoblast) Human Retinal Progenitor cells (RPE) Neuronal Stem Cell
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Stem and Progenitor Cell Expansion | Human embryonic stem cell (hESC) hMSCs (bone marrow derived, adipose derived) Human Retinal Progenitor cells (RPE) Neuronal Stem Cell
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In Vitro Differentiation of Pluripotent Stem Cells | hESC, hiPSC, mESC (Germ Cell Layers: ectoderm, mesoderm, endoderm; hematopoietic progenitor; definitive differentiation; cardiomyocytes) hESC, hiPSC (neuronal) hESC, hiPSC (smooth muscle) hESC, mESC, rESC (hepatocyte, hepatocyte-like) mESC, Chicken (cardiomyocytes) mESC, rESC, miPSC (neuronal, progenitor)
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In Vitro Differentiation of Adult Stem Cells | Hair Follicle (melanocytes, neurons, smooth muscle) MSC (cardiomyocyte, chondrocyte, hematopoetic, hepatocyte, neuron, osteocyte, spheroid)
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Featured Products, Resources, and More
Protocol: Fibronectin coated Transwell® Supports
Protocol: Fibronectin coated Transwell® Supports
Different cell lines require different fibronectin coating densities to achieve desired results. Refer to this protocol as a guide to get you started.
Different cell lines require different fibronectin coating densities to achieve desired results. Refer to this protocol as a guide to get you started.
Read More
Featured Products, Resources, and More
Corning 384 Well Clear Fibronectin Microplate
Corning 384 Well Clear Fibronectin Microplate
Learn more about the features and benefits of culturing cells on Corning 384-well clear Fibronectin-coated microplates.
Learn more about the features and benefits of culturing cells on Corning 384-well clear Fibronectin-coated microplates.
Read More
Featured Products, Resources, and More
Corning Surface Selection Guide
Corning Surface Selection Guide
Healthy cell growth relies on positive interactions between molecules and their microenvironment. Use this guide to choose the best surface for your specific application and cell type.
Healthy cell growth relies on positive interactions between molecules and their microenvironment. Use this guide to choose the best surface for your specific application and cell type.
Read MoreHow to Buy
Purchasing Options
Order Now
It’s easy to purchase Corning Fibronectin surfaces online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.
It’s easy to purchase Corning Fibronectin surfaces online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.
Customer Service
Customer Service
Contact UsIf you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.
If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.
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