ELISA Plates | Drug Discovery with ELISA Microplates | Corning

Cell-Based Assay Surfaces

Medium Binding Surface (Not-treated)

• Hydrophobic
• Ideal for large, hydrophobic biomolecules (>20kD)
• Binding capacity: ~250 ng IgG/cm²

Tissue Culture Treated Surface

• Hydrophilic and negatively charged due to incorporation of oxygen ions
• For attachment and growth of anchorage dependent cell lines

High Binding Surface

• Hydrophobic and ionic (negatively charged)
• Ideal for positively charged biomolecules (>10kD)
• Binding capacity: ~500 ng IgG/cm²

Corning CellBIND® Surface

• Hydrophilic and negatively charged
• Enhances cell attachment under difficult conditions
• Quickly adapts cells to reduced serum or serum-free conditions
• Improves overall attachment and yield

Corning DNA-BIND® Surface

• N-oxysuccinimide
• Allows covalent immobilization to amine groups
• Ideal for DNA, proteins, and small peptides

Corning BioCoat™ Surfaces

• Biological coatings of highly purified extracellular matrix (ECM) proteins Enhances attachment of transformed cell lines, transfected cells (including HEK-293), as well as a variety of primary and stem cells

• Helps cells stay attached during assay processing
• Surfaces include Poly-D-Lysine, Fibronectin, Collagen I, Gelatin,  Laminin, and Matrigel® Matrix

Corning Sulfhydryl-BIND Surface

• Maleimide
• Allows covalent immobilization to sulfhydryl groups
• Ideal for biomolecules possessing an accessible sulfhydryl group

Corning PureCoat™ Amine Surfaces

• Hydrophilic and ionic (positively charged)
• Ideal for small negatively charged biomoleculesFor covalent immobilization, use bifunctional crosslinkers

Carbo-BIND Surface

• Hydrazide
• Allows covalent immobilization to CHO moieties
• Ideal for antibodies and Fab fragments

Corning Nonbinding Surface (NBS)

• Non-ionic hydrophilic surface (polyethylene oxide-like)
• Inhibits hydrophobic and ionic interactions to reduce binding
• Enhances signal-to-noise in homogeneous assays